Towards Resolving the Interordinal Relationships of Placental Mammals.
Systematic Biology 48 (1): 1–5.
Here we show that progress towards a reliable phylogeny for placental mammals at the ordinal level continues apace. We draw especially upon insights from the recent “International Symposium on the Origin of Mammalian Orders” held at The Graduate University of Advanced Study, Hayama, Japan (21–25 July 1998), particularly work not incorporated in the remainder of this issue or published elsewhere. Abstracts to talks and posters presented at this meeting can be found at www.utexas.edu/ftp/depts/systbiol/ . The talks fell into three main sections, which we will now consider, followed by a summary where we present our current best estimate of the tree for placental mammals.
A Phylogenetic Foundation for Comparative Mammalian Genomics.
Genome Informatics 12: 141–154.
A major effort is being undertaken to sequence an array of mammalian genomes. Coincidentally, the evolutionary relationships of the 18 presently recognized orders of placental mammals are only just being resolved. In this work we construct and analyse the largest alignments of amino acid sequence data to date. Our findings allow us to set up a series of superordinal groups (clades) to act as prior hypotheses for further testing. Important findings include strong evidence for a clade of Euarchonta+ Glires (=Supraprimates) comprised of primates, flying lemurs, tree shrews, lagomorphs and rodents. In addition, there is good evidence for a clade of all placental mammals except Xenarthra and Afrotheria (=Boreotheria) and for the previously recognised clades Laurasiatheria, Scrotifera, Fereuungulata, Ferae, Afrotheria, Euarchonta, Glires, and Eulipotyphla. Accordingly, a revised classification of the placental mammals is put forward. Using this and molecular divergence-time methods, the ages of the superordinal splits are estimated. While results are strongly consistent with the earliest superordinal divergences all being > 65 mybp (Cretaceous period), they suffer from greater uncertainty than presently appreciated. The early primate split of tarsiers from the anthropoid lineage at ∼55 mybp is seen to be an especially informative fossil calibration point. A statistical framework for testing clades using SINE data is presented and reveals significant support for the tarsier/anthropoid clade, as well as the clades Cetruminantia and Whippomorpha. Results also underline our thesis that while sequence analysis can help set up hypothesised clades, SINEs obtainable from sequencing 1-2 MB regions of placental genomes are essential to testing them. In contrast, derivations suggest that empirical Bayesian methods for sequence data may not be robust estimators of clades. Our findings, including the study of genes such as TP53, make a good case for the tree shrew as a closer relative of primates than rodents, while also showing a slower rate of evolution in key cell cycle genes. Tree shrews are consequently high value experimental animals and a strong candidate for a genome sequencing initiative.
STANHOPE, M. J., WADDELL, V. G., MADSEN, O., DE JONG, W., BLAIR HEDGES, S., CLEVEN, G. C. , KAO, D. & SPRINGER, M. S. (1998)
Molecular evidence for multiple origins of Insectivora and for a new order of endemic African insectivore mammals.
PNAS 95 (17): 9967–9972. doi: 10.1073/pnas.95.17.9967.
The traditional views regarding the mammalian order Insectivora are that the group descended from a single common ancestor and that it is comprised of the following families: Soricidae (shrews), Tenrecidae (tenrecs), Solenodontidae (solenodons), Talpidae (moles), Erinaceidae (hedgehogs and gymnures), and Chrysochloridae (golden moles). Here we present a molecular analysis that includes representatives of all six families of insectivores, as well as 37 other taxa representing marsupials, monotremes, and all but two orders of placental mammals. These data come from complete sequences of the mitochondrial 12S rRNA, tRNA-Valine, and 16S rRNA genes (2.6 kb). A wide range of different methods of phylogenetic analysis groups the tenrecs and golden moles (both endemic to Africa) in an all-African superordinal clade comprised of elephants, sirenians, hyracoids, aardvark, and elephant shrews, to the exclusion of the other four remaining families of insectivores. Statistical analyses reject the idea of a monophyletic Insectivora as well as traditional concepts of the insectivore suborder Soricomorpha. These findings are supported by sequence analyses of several nuclear genes presented here: vWF, A2AB, and α-β hemoglobin. These results require that the order Insectivora be partitioned and that the two African families (golden moles and tenrecs) be placed in a new order. The African superordinal clade now includes six orders of placental mammals.
Throughout most of this century, the placental (eutherian) mammals with extant representation have been classified into 18 orders. During this period, the order Insectivora has been among the least stable higher taxa in Eutheria, both in terms of phylogenetic position and taxonomic content. Beginning with Huxley (1) and later embellished by Mathew (2), insectivores have been thought to possess features that rendered them closer to the ancestral stock of mammals. Despite this presumed central position of insectivores in the evolutionary history of mammals, the composition of the group never has been widely agreed on. The prevalent morphological view (3) suggests that the following extant families of “insectivores” descended from a single common ancestor and as such should be those groups that are regarded as the constituents of the order Insectivora (Lipotyphla): Soricidae (shrews), Tenrecidae (tenrecs), Solenodontidae (solenodons), Talpidae (moles), Erinaceidae (hedgehogs and gymnures), and Chrysochloridae (golden moles).
Butler (4) listed six morphological characteristics that, in his opinion, supported a monophyletic Insectivora including (i) absence of cecum; (ii) reduction of pubic symphysis; (iii) maxillary expansion within orbit, displacing palatine; (iv) mobile proboscis; (v) reduction of jugal; and (vi) hemochorial placenta. More recently, MacPhee and Novacek (3) have reviewed the evidence and concluded that characteristics (i) and (ii) support lipotyphlan monophyly, characteristic (iii) possibly does, and (iv–vi), as currently defined, do not, leaving two to three characteristics that, in their opinion, support the order Insectivora.
The six families of insectivores are most often grouped into two clades of subordinal rank: the Erinaceomorpha (hedgehogs) and the Soricomorpha (all other families). Within the Soricomorpha, Butler (4) suggested that the golden moles and tenrecs form a clade and that moles and shrews cluster together, followed by solenodons. MacPhee and Novacek (3), however, proposed three clades of subordinal rank: Chrysochloromorpha (Chrysochloridae), Erinaceomorpha (Erinaceidae), and Soricomorpha (Soricidae, Talpidae, Solenodontidae, and Tenrecidae). This latter organization is based on their view that the Chrysochloridae is “spectacularly autapomorphic.” In their opinion, golden moles show no shared derived traits with the soricomorphs and therefore should be separated from that suborder. This recommendation echoes earlier views regarding the group that have suggested that golden moles are a separate order or suborder (5–7).
A recent molecular study of mammalian phylogeny, which included three insectivore families, demonstrated that golden moles are not part of the Insectivora but instead belong to a clade of endemic African mammals that also includes elephants, hyraxes, sea cows, aardvarks, and elephant shrews (8). Evidence for this now comes from a wide range of disparate molecular loci including the nuclear AQP2, vWF, and A2AB genes as well as the mitochondrial 12S–16S rRNA genes (8, 9). The fossil record of golden moles indicates that the geographic distribution of this group has been restricted to Africa throughout its temporal range (10, 11). The fossil record of tenrecs also suggests an African origin (10, 11). This paleontological record, along with the morphological study of Butler (4), suggests a possible common ancestry for golden moles and tenrecs. However, it also may be that the autapomorphic qualities of golden moles reflect their evolutionary history as a singular distinct lineage of African insectivores, separate from the rest of the order. At present, there is no published molecular phylogenetic perspective on the evolutionary history of the Tenrecidae, there is no molecular study that includes a representative from all families of insectivores, and there are no molecular sequence data (data banks or published accounts) for solenodons.
Here, we report a molecular phylogenetic analysis involving all families of insectivores, using complete sequences of the mitochondrial 12S rRNA, 16S rRNA, and tRNA-Valine genes. These data, along with additional sequences from four disparate nuclear genes, are used to examine the extent of insectivore paraphyly or polyphyly, the conflicting hypotheses regarding the origin of the family Tenrecidae, and the possibility of an African clade of insectivores.
SPRINGER, M. S., CLEVEN, G. C. , MADSEN, O., DE JONG, W WADDELL, V. G., AMRINE, H. M., & STANHOPE, M. J. (1997)
Nature 388: 61-64 (3 July 1997)
Letter to Nature:
The order Insectivora, including living taxa (lipotyphlans) and archaic fossil forms, is central to the question of higher-level relationships among placental mammals1. Beginning with Huxley, it has been argued that insectivores retain many primitive features and are closer to the ancestral stock of mammals than are other living groups. Nevertheless, cladistic analysis suggests that living insectivores, at least, are united by derived anatomical features. Here we analyse DNA sequences from three mitochondrial genes and two nuclear genes to examine relationships of insectivores to other mammals. The representative insectivores are not monophyletic in any of our analyses. Rather, golden moles are included in a clade that contains hyraxes, manatees, elephants, elephant shrews and aardvarks. Members of this group are of presumed African origin. This implies that there was an extensive African radiation from a single common ancestor that gave rise to ecologically divergent adaptive types. 12S ribosomal RNA transversions suggest that the base of this radiation occurred during Africa's window of isolation in the Cretaceous period before land connections were developed with Europe in the early Cenozoic era.
Relationships of Cetacea (Artiodactyla) Among Mammals: Increased Taxon Sampling Alters Interpretations of Key Fossils and Character Evolution.
PLoS ONE 4(9): e7062. doi:10.1371/journal.pone.0007062.
Integration of diverse data (molecules, fossils) provides the most robust test of the phylogeny of cetaceans. Positioning key fossils is critical for reconstructing the character change from life on land to life in the water.
We reexamine relationships of critical extinct taxa that impact our understanding of the origin of Cetacea. We do this in the context of the largest total evidence analysis of morphological and molecular information for Artiodactyla (661 phenotypic characters and 46,587 molecular characters, coded for 33 extant and 48 extinct taxa). We score morphological data for Carnivoramorpha, †Creodonta, Lipotyphla, and the †raoellid artiodactylan †Indohyus and concentrate on determining which fossils are positioned along stem lineages to major artiodactylan crown clades. Shortest trees place Cetacea within Artiodactyla and close to †Indohyus, with †Mesonychia outside of Artiodactyla. The relationships of †Mesonychia and †Indohyus are highly unstable, however - in trees only two steps longer than minimum length, †Mesonychia falls inside Artiodactyla and displaces †Indohyus from a position close to Cetacea. Trees based only on data that fossilize continue to show the classic arrangement of relationships within Artiodactyla with Cetacea grouping outside the clade, a signal incongruent with the molecular data that dominate the total evidence result.
Integration of new fossil material of †Indohyus impacts placement of another extinct clade †Mesonychia, pushing it much farther down the tree. The phylogenetic position of †Indohyus suggests that the cetacean stem lineage included herbivorous and carnivorous aquatic species. We also conclude that extinct members of Cetancodonta (whales + hippopotamids) shared a derived ability to hear underwater sounds, even though several cetancodontans lack a pachyostotic auditory bulla. We revise the taxonomy of living and extinct artiodactylans and propose explicit node and stem-based definitions for the ingroup.
The principles of classification and a classification of mammals.
Bulletin of the American Museum of Natural History 85.
George Gaylord SIMPSON war Kurator für fossile Säugetiere und Vögel am Amerikanischen Museum für Naturkunde in New York. Die von ihm begründete Klassifizierung der Säugetiere hatte ein halbes Jahrhundert Bestand und wurde während dieser Zeit im Wesentlichen von den säugetierkundlichen Standardwerken übernommen. Im deutschsprachigen Raum wurde diese Systematik vor allem durch eine Veröffentlichung von MÜLLER-USING und HALTENORTH aus dem Jahr 1954, die in den „Säugetierkundlichen
Mitteilungen" auf der Basis der SIMPSON’schen Klassifikation für die rezenten 22 Unter- und 10 Teilordnungen sowie für die 52 Überfamilien und 118 Familien der Säugetiere deutsche Namen vorschlugen
O'LEARY, M. A., BLOCH, J. I., FLYNN, J. J., GAUDIN, T. J., GIALLOMBARDO, A., GIANNINI, N. P., GOLDBERG, S. L., KRAATZ, B. P., LUO, Z.-X., MENG, J., NI, X., NOVACEK, M. J PERINI, F. A., RANDALL, Z. S., ROUGIER, G. W., SARGIS, E. J., SILCOX, M. T., SIMMONS, N. B., SPAULDING, M., VELAZCO, P. M., WEKSLER, M., WIBLE, J. R. & CIRRANELLO, A. L. (2013)
The Placental Mammal Ancestor and the Post–K-Pg Radiation of Placentals.
Science (08 Feb 2013) 339 (6120): 662-667.
To discover interordinal relationships of living and fossil placental mammals and the time of origin of placentals relative to the Cretaceous-Paleogene (K-Pg) boundary, we scored 4541 phenomic characters de novo for 86 fossil and living species. Combining these data with molecular sequences, we obtained a phylogenetic tree that, when calibrated with fossils, shows that crown clade Placentalia and placental orders originated after the K-Pg boundary. Many nodes discovered using molecular data are upheld, but phenomic signals overturn molecular signals to show Sundatheria (Dermoptera + Scandentia) as the sister taxon of Primates, a close link between Proboscidea (elephants) and Sirenia (sea cows), and the monophyly of echolocating Chiroptera (bats). Our tree suggests that Placentalia first split into Xenarthra and Epitheria; extinct New World species are the oldest members of Afrotheria.
The Phylogenetic Position of the Talpidae Within Eutheria Based on Analysis of Complete Mitochondrial Sequences.
Molecular Biology and Evolution. 17 (1): 60–67.
The complete mitochondrial (mt) genome of the mole Talpa europaea was sequenced and included in phylogenetic analyses together with another lipotyphlan (insectivore) species, the hedgehog Erinaceus europaeus, and 22 other eutherian species plus three outgroup taxa (two marsupials and a monotreme). The phylogenetic analyses reconstructed a sister group relationship between the mole and the fruit bat Artibeus jamaicensis (order Chiroptera). The Talpa/Artibeus clade constitutes a sister clade of the cetferungulates, a clade including Cetacea, Artiodactyla, Perissodactyla, and Carnivora. A monophyletic relationship between the hedgehog and the mole was significantly rejected by maximum parsimony and maximum likelihood. Consistent with current systematic schemes, analyses of complete cytochrome b genes including the shrew Sorex araneus (family Soricidae) revealed a close relationship between Talpidae and Soricidae. The analyses of complete mtDNAs, along with the findings of other insectivore studies, challenge the maintenance of the order Lipotyphla as a taxonomic unit and support the elevation of the Soricomorpha (with the families Talpidae and Soricidae and possibly also the Solenodontidae and Tenrecidae) to the level of an order, as previously proposed in some morphological studies.
Phylogenetic relationships of artiodactyls and cetaceans as deduced from the comparison of cytochrome b and 12S rRNA mitochondrial sequences.
Mol. Biol. Evol. (1997) 14 (5): 550-559.
A data set of complete mitochondrial cytochrome b and 12S rDNA sequences is presented here for 17 representatives of Artiodactyla and Cetacea, together with potential outgroups (two Perissodactyla, two Carnivora, two Tethytheria, four Rodentia, and two Marsupialia). We include seven sequences not previously published from Hippopotamidae (Ancodonta) and Camelidae (Tylopoda), yielding a total of nearly 2.1 kb for both genes combined. Distance and parsimony analyses of each gene indicate that 11 clades are well supported, including the artiodactyl taxa Pecora, Ruminantia (with low 12S rRNA support), Tylopoda, Suina, and Ancodonta, as well as Cetacea, Perissodactyla, Carnivora, Tethytheria, Muridae, and Caviomorpha. Neither the cytochrome b nor the 12S rDNA genes resolve the relationships between these major clades. The combined analysis of the two genes suggests a monophyletic Cetacea +Artiodactyla clade (defined as "Cetartiodactyla"), whereas Perissodactyla, Carnivora, and Tethytheria fall outside this clade. Perissodactyla could represent the sister taxon of Cetartiodactyla, as deduced from resampling studies among outgroup lineages. Cetartiodactyla includes five major lineages: Ruminantia, Tylopoda, Suina, Ancodonta, and Cetacea, among which the phylogenetic relationships are not resolved. Thus, Suiformes do not appear to be monophyletic, justifying their split into the Suina and Ancodonta infraorders. An association between Cetacea and Hippopotamidae is supported by the cytochrome b gene but not by the 12S rRNA gene. Calculation of divergence dates suggests that the Cetartiodactyla could have diverged from other Ferungulata about 60 MYA.
Molekularbiologische Untersuchungen zur Phylogenie der Hornvögel (Aves: Bucerotiformes).
ca. 57 Seiten
Fachbereich Biologie, Johann Wolfgang Goethe-Universität Frankfurt am Main
Zusammenfassung: siehe hier